Flow binding instruction manual

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Note: Dynamic binding capacity will vary from protein to protein. ** Maximum pressure test: Profinity IMAC resin packed in a x 30 cm Amicon column to a bed height of 20 cm with 20 mM sodium phosphate buffer up to 43 psi (3 bar). Flow rates were increased stepwise by cm/hr and held for 2 min at each step. Flow rate: ml/min or 1 ml/min Binding buffer: 20 mM sodium phosphate, M NaCl, 5 mM imidazole, pH Elution buffer: 20 mM sodium phosphate, M NaCl, M imidazole, pH Note: Dynamic binding capacity is protein-dependent. †† H 2O at room temperature §§§ Ni2+-stripped medium. Observed dynamic binding capacity IgG 25–60 mg IgG/ml CHT* 15–25 mg IgG/ml CHT** Typical linear flow rate range 50–1, cm/hr pH stability*** –14 pH Base stability at least 21 months in 1 N NaOH Regeneration mM sodium phosphate, pH 7 1, mM trisodium phosphate, pH 11–

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